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BinaxNOW® G6PD

BinaxNOW® G6PD Test is a rapid test for the qualitative detection of G6PD enzyme activity in human venous whole blood. The simple, three step test provides results in less than 10 minutes, allowing for prompt treatment decisions.

Intended Use

The BinaxNOW® G6PD (Glucose-6-Phosphate Dehydrogenase) Test is an in vitro enzyme chromatographic test for the qualitative detection of G6PD enzyme activity in human venous whole blood, collected in heparin or ethylenediaminetetraacetic acid (EDTA).

The BinaxNOW® G6PD Test is a visual screening test used for differentiating normal from deficient G6PD activity levels in whole blood and is intended to aid in the identification of people with G6PD deficiency. Samples which generate deficient results should be assayed using a quantitative G6PD test method to verify the deficiency.

Product details

BinaxNOW® G6PD
Sample type: Venous Whole blood
Time to result:

Heparin treated samples: 5 minutes

  EDTA treated samples: 7 minutes
Kit size: 25 tests
Performance data*: Heparin samples
  Deficient result percent agreement: 98%
  Normal result percent agreement: 97.9%
Peformance data: EDTA samples
  Deficient result percent agreement: 98.0%
  Normal result percent agreement: 97.4%
 Regulatory: Cleared for distribution by the FDA
  Not CE marked
  Available in english

*compared to a commercially available quantitative G6PD test

For instructions for use, see package insert

About G6PD deficiency

G6PD is an enzyme that is part of the hexose monophosphate shunt and is the first enzyme of the pentose pathway. The enzyme is involved in the catalytic conversion of glucose to 6 phospho-gluconate, which produces an energy equivalent (NADPH) in the process.

G6PD deficiency is the largest and most widespread enzymopathy in the world, affecting some 200 million people. There are approximately 400 variants and deficiency of the enzyme is frequently seen in males. The highest known gene frequency is 0.65 among Kurdish Jews. Prevalence is approximately 21% in West Africa and 11% in some Asian countries such as Thailand1. In middle and northern Europe the frequency of G6PD deficiency is about 0.0005. In the United States, the gene frequency of enzyme deficiency is 10 - 11% among African American males2.

Although much of the research on G6PD has focused on its function in red blood cells and its importance in cellular metabolism, it is equally important in providing defense mechanisms for erythrocyte membranes against oxidative stress.

When strong oxidizing agents such as those found in many commonly used drugs (anti-malarial drugs, sulfa drugs, and ascorbic acid) 3 are administered, a deficiency in red cell G6PD does not allow for the production of sufficient reducing equivalents to prevent clinical complications such as acute spherocytic hemolytic anemia. It is therefore important that individuals with this deficiency be identified prior to the use of certain therapeutic agents.

For more information about G6PD deficiency, please see the links below:

G6PD deficiency website: http://g6pddeficiency.org/
G6PD deficiency favism association http://www.g6pd.org/

References

1. Erbağci A.B. and N. Yilmaz. 2002. Erythrocyte Glucose 6-Phosphate Dehydrogenase Deficiency Frequency in Gaziantep, Turkey. Eastern Journal of Medicine 7 (1): 15-18.

2. Ernest Beutler. 1994. G6PD Deficiency. Blood 84 (11): 3613-3636.

3. Stiene EA. 1972. Red cell enzyme deficiencies: A Review. Am J Med Tech.38:454.

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BinaxNOW® G6PD Specifications 

BinaxNOW® G6PD Test

Product code: 780-000
Number of Tests: 25

BinaxNOW® G6PD Resources

Brochure      

  Multilingual
  Package Insert

      Procedure Card
G6PD Brochure Thumbnail       G6PD Package Insert Thumbnail       G6PD Procedure Card

Material Safety Data Sheets


Frequently Asked Questions

What should I do if the sample front does not completely cover the top of the reaction pad?

This represents an invalid result. Do not use the test result. Repeat with a new test device.

Can test results be read after 5 (heparin) /7 (EDTA) minutes?

No. Read all test results as close to their designated read times (5 or 7 minutes) as possible.

Can the test be used with venous blood treated with anticoagulants other than EDTA or Heparin?

No. Heparin and EDTA are the only anticoagulants that have been evaluated for use with this test.

Why can’t the test devices be exposed to any sunlight or to bright fluorescent lights for longer than 5 minutes, prior to testing?

The reagents on the test strip are light sensitive, so the test device must be sealed in its foil pouch until just before use. Once removed from the pouch, do not expose the device to direct sunlight or perform the test near a sunny window. Do not expose the device to fluorescent light for longer than 5 minutes, prior to testing.

If the temperature in the testing lab is not within the range of 18-25oC (64ºF to 77ºF), can I still run the BinaxNOW G6PD test?

No – The test method utilizes a kinetic enzymatic reaction, which is affected by temperature. The test must be performed at temperatures between 18-25oC (64ºF to 77ºF); failure to perform testing in the specified temperature range could lead to erroneous results. If the temperature is outside this range, DO NOT PERFORM THE TEST.